ORIGINAL ABSTRACT

Glyphosate is the most used herbicide on the planet because of its excellent efficacy on almost all weed species and due to the large-scale adoption of transgenic, glyphosate-resistant (GR) crops. Agnes Rimando became an expert in glyphosate analysis almost 20 years ago to support research on GR crop safety and on mechanisms of evolved glyphosate resistance by weeds. Her work was the first to show that the amount of glyphosate and its primary metabolite aminomethylphosphonic acid (AMPA) that accumulates in GR soybean seed from plants treated with approved glyphosate doses can approach their legal limits. However, she later found that only trace amounts of these compounds accumulate in the seed of GR maize treated with recommended glyphosate doses. She showed that GR canola, the only transgenic crop with a transgene encoding an enzyme for degradation of glyphosate, metabolizes glyphosate to AMPA very rapidly. Her work was instrumental in providing support for the view that "yellow flash" symptoms sometimes observed in field-grown GR soybeans are due to accumulation of enough AMPA to cause mild phytotoxicity. She did the chemical analyses in the only paper to survey the capacity of an array of plant species to metabolize glyphosate to AMPA. She found a wide range in this capacity, with grasses with little or no metabolism of glyphosate to AMPA and with legumes readily metabolizing glyphosate. Lastly, she found no evidence of enhanced degradation of glyphosate to be a mechanism of evolved resistance to glyphosate by two weed species but that it might be involved in natural tolerance to glyphosate of some weeds.