ORIGINAL ABSTRACT

Pesticide poisoning constantly threatens bees as they forage for resources in pesticide-treated crops. This poisoning requires thorough investigation to identify its causes, underscoring the importance of reliable pesticide detection methods for bee monitoring. Infrared spectroscopy provides reflectance data across hundreds of spectral bands (hyperspectral reflectance), presumably enabling the efficient classification of pesticide contamination in bee carcasses using artificial intelligence (AI) models, such as machine learning. In this study, bee contamination by commercial formulations of three insecticides-dimethoate (organophosphate), fipronil (phenylpyrazole), and imidacloprid (neonicotinoid)-as well as glyphosate, the most widely used herbicide globally, was detected using machine learning models. These models classified the hyperspectral reflectance profiles of the body surfaces of contaminated bees. The best-performing model, the linear discriminant analysis, achieved 98 % accuracy in discriminating contamination across species Apis mellifera, Melipona mondury, and Partamona helleri, with prediction speeds of 0.27 s. Our pioneering study introduced an effective method for discerning multiple classes of bees contaminated with pesticides using hyperspectral reflectance. An AI-driven spectral data analysis tool (https://github.com/bernardesrodrigoc/MACSS) was developed for the purpose of identifying and characterizing new samples through their spectral characteristics. This platform aids efforts to monitor and conserve bee populations and holds potential importance in environmental monitoring, agricultural research, and industrial quality control.