ORIGINAL ABSTRACT

N-(phosphonomethyl)glycine (glyphosate) is the most widely used herbicide worldwide. Although it has been extensively studied, few studies use realistic environmental concentrations to assess its potential effects on fish embryos and larvae. This work aims to evaluate potential neurotoxic and reproductive effects of realistic concentrations of glyphosate in non-target aquatic species using zebrafish larvae. Biological and reproductive biomarkers (condition factor, hepatic and gonadic indices, and fertility rate) were evaluated for adults exposed to 0, 10, 100, and 1000 µg/L, while a transcriptomic comparison was carried out for larvae from both exposure scenarios at 1000 µg/L. The fertility rate of exposed parents decreased with increasing glyphosate concentration, while gonadosomatic (GSI) and hepatosomatic (HIS) indices of females treated with 100 µg/L glyphosate were significantly higher in glyphosate-exposed fish compared to the control group; however, glyphosate treatment did not significantly change GSI or HSI in males. Transcriptomic analysis in larvae showed that glyphosate could alter developmental and metabolic processes, targeting the nervous system in both exposure schemes. The ability of glyphosate to alter the development of the nervous system in larvae of exposed parents suggests that exposure to gametes could produce intergenerational alterations, with potential ecotoxicological implications that remain to be determined.